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Enalapril microbial biosensor
S Fleschin1, C Bala, A A Bunaciu
1Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, Romania.
Preparative Biochemistry & Biotechnology
|August 26, 1998
Summary
A new microbial biosensor was developed using Bacillus subtilis to detect enalapril maleate, an ACE inhibitor. This biosensor accurately measures the drug
Area of Science:
- Analytical Chemistry
- Biotechnology
- Microbiology
Background:
- Enalapril maleate (EMa) is a key antihypertensive drug belonging to the angiotensin-converting enzyme (ACE) inhibitor class.
- Accurate quantification of EMa is crucial for pharmaceutical quality control and therapeutic efficacy.
Purpose of the Study:
- To develop and validate a novel microbial biosensor for the detection and quantification of enalapril maleate.
- To utilize induced Bacillus subtilis cells for a sensitive and specific EMa measurement.
Main Methods:
- Development of a microbial biosensor employing induced Bacillus subtilis.
- Measurement of the drug's effect on the respiration rate of the microbial cells.
- Application of the biosensor for the determination of EMa in pharmaceutical formulations.
Main Results:
- Successful development of a microbial biosensor for enalapril maleate.
- The biosensor effectively measures the acceleration of respiration linked to EMa metabolism.
- Good results were obtained when applied to determine the active ingredient in pharmaceutical tablets.
Conclusions:
- A Bacillus subtilis-based microbial biosensor offers a viable method for enalapril maleate determination.
- This biosensor provides a sensitive and efficient approach for quality control of enalapril maleate in pharmaceutical products.