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Growth factor regulation of insulin-like growth factor binding protein secretion by cultured human granulosa-luteal
O W Yap1, Y A Chandrasekher, L C Giudice
1Department of Gynecology and Obstetrics, Stanford University Medical Center, California 94305, USA.
Objective:
To examine the effects of epidermal growth factor (EGF), transforming growth factor-beta (TGF-beta), and fibroblast growth factor (FGF) on insulin-like growth factor binding protein (IGFBP) secretion by cultured human granulosa-luteal cells.
Design:
Granulosa-luteal cells obtained at the time of oocyte harvest for IVF were cultured in serum-free medium in the presence or absence of EGF, TGF-beta, or FGF. Conditioned medium then was analyzed by Western ligand blot and immunoradiometric assays.
Setting:
An academic medical center.
Patient(S):
Women undergoing IVF.
Intervention(S):
None.
Main Outcome Measure(S):
IGFBP-1 secretion.
Result(S):
By Western ligand blot analysis, IGFBP-1 levels were 1.4-fold to 7.4-fold higher in conditioned medium from cells cultured in the presence of EGF than in control medium. By immunoradiometric assay, IGFBP-1 levels increased from 1.6 to 9.9 times over control. The TGF-beta had no apparent effect, and FGF did not consistently stimulate IGFBP-1 secretion.
Conclusion(S):
The EGF may decrease intrafollicular bioavailable IGF levels by increasing inhibitory IGFBPs, thereby leading to arrest of follicular development. Interactions between the EGF and IGF systems may be involved in the processes governing human ovarian follicle maturation and atresia.