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Related Experiment Videos

Surface topography can interfere with epithelial tissue migration

J H Fitton1, B A Dalton, G Beumer

  • 1CSIRO Molecular Science and Cooperative Research Centre for Eye Research and Technology, Riverside Corporate Park, NSW, Australia.

Journal of Biomedical Materials Research
|October 17, 1998
PubMed
Summary
This summary is machine-generated.

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Synthetic polymer pore size significantly inhibits corneal epithelial tissue migration. Larger pores, especially above 0.9 micrometers, halt tissue outgrowth, impacting wound healing research.

Area of Science:

  • Biomaterials Science
  • Ophthalmology
  • Cell Biology

Background:

  • Corneal epithelial wound healing involves cell migration.
  • Synthetic polymer scaffolds are used in regenerative medicine.
  • Substrate topography can influence cell behavior.

Purpose of the Study:

  • To investigate the effect of synthetic substrate porosity on corneal epithelial tissue migration.
  • To determine the critical pore size that inhibits epithelial outgrowth.
  • To compare the migration response of stratified epithelium versus dissociated cells.

Main Methods:

  • In vitro study using cellulose nitrate/acetate and track-etched polycarbonate membranes.
  • Varying pore sizes (up to 2.3 micrometers) and densities were tested.

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  • Corneal epithelial tissue outgrowth and dissociated cell migration were assessed.
  • Effect of fibronectin and collagen coating was evaluated.

    • Main Results:

    • Increased pore size inhibited corneal epithelial tissue outgrowth.
    • Outgrowth was completely halted on membranes with mean pore diameters of 0.9 micrometers.
    • Coating with fibronectin or collagen partially rescued outgrowth on smaller pores (<0.9 micrometers).
    • Dissociated cell migration was reduced but not completely inhibited by pores up to 2.3 micrometers.

    Conclusions:

    • Specific synthetic surface topographies, particularly pore size, can inhibit adult stratified corneal epithelial tissue movement.
    • Epithelial tissue outgrowth is more sensitive to pore size inhibition than dissociated cell migration.
    • Findings have implications for designing biomaterials for corneal regeneration.