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Related Experiment Videos

Coronavirus envelope glycoprotein assembly complexes

V P Nguyen1, B G Hogue

  • 1Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030, USA.

Advances in Experimental Medicine and Biology
|October 23, 1998
PubMed
Summary

Coronaviruses assemble via protein complexes. This study found similar spike (S) and membrane (M) glycoprotein complexes in mouse hepatitis coronavirus A59 and bovine coronavirus infected cells, crucial for virus assembly.

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Area of Science:

  • Virology
  • Molecular Biology
  • Cell Biology

Background:

  • Protein:protein interactions and subcellular localization are critical for coronavirus assembly.
  • Envelope glycoproteins, including spike (S), membrane (M), and hemagglutinin esterase (HE), are key components of coronaviruses.

Purpose of the Study:

  • To identify and characterize glycoprotein complexes involved in the assembly of mouse hepatitis coronavirus A59 (MHV-A59) and bovine coronavirus (BCV).
  • To investigate the temporal association and subcellular localization of these glycoprotein complexes.

Main Methods:

  • Immunoprecipitation assays to identify protein complexes.
  • Metabolic labeling and pulse-chase experiments for kinetic analyses.
  • Analysis of infected cells using microscopy and biochemical techniques.

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Main Results:

  • Similar complexes of spike (S) and membrane (M) proteins were identified in MHV-A59 and BCV infected cells.
  • S and M proteins rapidly associated post-translation, likely initiating interaction at a pre-Golgi compartment.
  • The hemagglutinin esterase (HE) protein was found to be part of a complex with M and S proteins in BCV infected cells.

Conclusions:

  • The findings suggest that conserved glycoprotein complexes are essential for the assembly of different coronaviruses.
  • These prerequisite complexes play a fundamental role in the viral assembly process.