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[The localization of intermediate filaments in polykaryons in the interphase and mitosis]

G E Onishchenko, O M Petrashchuk

    Tsitologiia
    |January 1, 1994
    PubMed

    Insights

    Vimentin intermediate filaments form distinct networks around each nucleus in fused cells. This compartmentalization is maintained through mitosis until anaphase, when the central network disassembles, aiding cell division.

    Area of Science:

    • Cell Biology
    • Cytoskeleton Dynamics
    • Intermediate Filaments

    Context:

    • Investigating the behavior of vimentin intermediate filaments in multinucleated cells (polykaryons) formed by cell fusion.
    • Utilizing Chinese hamster cells (line Ag17) for experimental observation.

    Purpose:

    • To determine the localization and behavior of vimentin filament networks during the cell cycle, particularly mitosis, in fused cells.
    • To understand the role of intermediate filaments in the distribution of cellular components during cell division.

    Summary:

    • Vimentin filaments form a network around each nucleus in interphase polykaryons.
    • This compartmentalization persists through early mitosis (up to metaphase) in both bipolar and multipolar cells.
    • During anaphase, the central vimentin network disassembles, leaving a peripheral network, and reorganization begins in telophase, concluding in daughter cells.

    Impact:

    • Provides insights into the dynamic organization of the cytoskeleton during mitosis in multinucleated cells.
    • Suggests a role for intermediate filaments in the segregation of chromosomes and centrioles during cell division.
    • Contributes to understanding the structural basis of cell division mechanics.

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