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Updated: Jul 15, 2026
11:48
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
Published on: October 9, 2014
代替的なプレ-mRNAスプライシングの単分子プロファイリング
Jun Zhu1, Jay Shendure, Robi D Mitra
1Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
Science (New York, N.Y.)
|August 9, 2003
まとめ
デジタルポロニエエクソンプロフィーリングは,代替的な前メッセンジャーRNAスプライシングの研究のための新しい技術です. これは,研究者が個々のトランスクリプトの多様なスプライシングパターンを監視し,定量化することを可能にします.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオインフォマティックス
背景:
- オルタナティブ・プレメッセンジャーRNA (mRNA) スプライシングはタンパク質の多様性を生み出し,哺乳類の複雑さに寄与する.
- 代替スプライシングの理解は,限られた遺伝子セットから遺伝子機能と生物の複雑さを説明するために不可欠です.
研究 の 目的:
- デジタルポロニーエクソンプロファイリングと呼ばれる単一分子ベースの新しいテクノロジーを紹介し,説明する.
- 個々のトランスクリプトレベルでの複雑な代替的な前メッセンジャーRNAスプライシングパターンの研究を可能にする.
主な方法:
- デジタルポロニウムエクソンプロフィーリングは,単一ヌクレオチド解像度のためのミニシーケンシング戦略を使用します.
- この技術は,個々のスプライシングのバリエーションを定量化するためにデジタルアプローチを採用しています.
- これは,単一のトランスクリプト内のエクソン含有における組み合わせ多様性の監視を可能にします.
主要な成果:
- 開発された技術は,代替スプライシングイベントの高解像度分析を提供します.
- それは,サンプルに存在する異なるスプライシングの変数を正確に定量化することを可能にします.
- 単一分子レベルでエクソンインクルージョンの多様性を研究する能力を実証します.
結論:
- デジタルポロニエエクソンプロファイリングは,複雑な代替スプライシングを調査するための強力なツールです.
- この技術は,代替的にスプライスされたmRNAの生理学的および病理学的役割を研究するために適用できます.
- それは,代替スプライシングを規制するメカニズムを理解するための新しい道を提供します.
関連する概念動画
01:32RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
02:01Pre-mRNA Processing
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
01:32RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
02:18Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
01:32Pre-mRNA Processing: RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
02:18Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...