関連する実験動画
Updated: Jul 10, 2026
11:56
Generation of Mice Derived from Induced Pluripotent Stem Cells
Published on: November 29, 2012
人間の体細胞から派生した誘導性多能幹細胞系
Junying Yu1, Maxim A Vodyanik, Kim Smuga-Otto
1Genome Center of Wisconsin, Madison, WI 53706-1580, USA. jyu@primate.wisc.edu
Science (New York, N.Y.)
|November 22, 2007
まとめ
4つの重要な要因は,ヒトの体細胞を胚性幹細胞を模倣して,誘導された多能幹細胞に再プログラムします. これらの細胞は,疾患モデリングと再生医療に期待を寄せている.
科学分野:
- 幹細胞生物学 幹細胞生物学とは
- 再プログラム技術を再プログラムする.
- 人間の細胞の微分化
背景:
- ソマティック細胞核移転 (SCNT) は,オオサイト因子を利用してソマティック細胞を分化します.
- 以前,多能幹細胞を生成する方法は複雑で限界がありました.
研究 の 目的:
- 人間の体細胞を再プログラムするのに十分な最小限の要素のセットを特定する.
- その結果生じる誘発性多能幹細胞の性質を特徴付けるため.
主な方法:
- 人間の体細胞に4つの特定の転写因子 (OCT4,SOX2,NANOG,LIN28) を導入した.
- プラリポテンシーマーカー,カリタイプ,テロメラーゼ活性,および差別化ポテンシャルの評価.
主要な成果:
- ソマティック細胞から誘発性多能性ヒト幹細胞 (iPSC) を成功裏に生成しました.
- これらのiPSCは,正常なカリオタイプと分化能力を含む,ヒト胚性幹細胞 (ES) の特徴を示した.
- 再プログラムされた細胞は,重要なES細胞マーカーと遺伝子を発現し,テロメラーゼ活性を示した.
結論:
- 4つの要因の定義されたセットは,人間の体細胞に多能性を誘発するのに十分です.
- 生成されたiPSCは,ヒトのES細胞に似た特性と発達可能性を備えています.
- これらのiPSCは,技術的な課題が解決されるまで,疾患モデリング,薬剤発見,移植治療の可能性を秘めています.
関連する概念動画
01:06Induced Pluripotent Stem Cells
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Somatic cells are...
01:13Induced Pluripotent Stem Cells
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
01:13Induced Pluripotent Stem Cells
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
01:22iPS Cell Differentiation
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
01:29Somatic to iPS Cell Reprogramming
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
01:21EPS and iPS Cells in Disease Research
Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...