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激发性动力学和依赖于雅普的机械线索驱动了细分时钟
Alexis Hubaud1, Ido Regev2, L Mahadevan3
1Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS (UMR 7104), Inserm U964, Université de Strasbourg, Illkirch 67400, France; Department of Genetics, Harvard Medical School and Department of Pathology, Brigham and Women's Hospital, 60 Fenwood Road, Boston, MA 02115, USA.
Cell
|September 26, 2017
概括
脊椎动物的细分依赖于细分时钟. 这项研究表明, 振荡是细胞的共同属性,
科学领域:
- 发育生物学
- 细胞动力学
- 遗传学
背景情况:
- 脊椎动物的身体轴分为体和脊椎取决于分段时钟.
- 细分时钟涉及前皮间皮 (PSM) 中的节奏信号通路活动.
- 目前尚不清楚这些振荡是单个细胞内在的还是群体现象.
研究的目的:
- 调查分段时钟振荡是单细胞还是群体级别的属性.
- 建立体外培养条件以观察稳定的细胞振荡.
- 探索量子感应和机械线索在触发这些振荡中的作用.
主要方法:
- 开发了体外培养条件以实现稳定的细胞振荡.
- 使用Yap和Notch信号通道来触发振荡.
- 操纵了依赖于雅普的机械线索来改变细胞状态.
主要成果:
- 证明分段时钟振荡是PSM细胞的集体属性.
- 通过动态定数传感信号可以在体外激发振荡.
- 证实操纵Yap依赖的机械线索可以将孤立的PSM细胞从静止状态转换为振荡状态.
结论:
- 细分时钟的功能是一个可激发的系统.
- 振荡是一种细胞的集体行为, 不仅仅是单细胞的内在特性.
- 介绍了研究脊椎动物形态变异动态的新范式.
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