电弧诱导喷射电离电离化质谱测量
Kaineng Huang1, Hui Zeng1, Xingyue Li1
1College of Chemistry and Materials Science, Sichuan Normal University, Chengdu, Sichuan 610068, P. R. China.
Analytical chemistry
|December 28, 2023
概括
弧式诱导电喷电离质谱 (AESI-MS) 提供了一种新的软电离技术. 这种方法简化了质谱,并提高了包括氨基酸在内的各种分析物的电离效率.
科学领域:
- 分析化学 分析化学
- 质谱测量质量谱测量
- 等离子体物理学的物理学
背景情况:
- 传统的电子喷雾电离 (ESI) 面临着复杂的矩阵和某些分析物类型的局限性.
- 大气压化学电离 (APCI) 和ESI是已知的软电离方法.
- 开发新的电离技术对于扩大质谱应用至关重要.
研究的目的:
- 介绍和描述弧度诱导电喷射电离质谱法 (AESI-MS) 作为一种新的软电离技术.
- 为了证明AESI-MS在各种分析挑战中比传统ESI更有优势.
- 探索AESI-MS用于分析各种化合物的潜力,包括复杂样品中的氨基酸.
主要方法:
- 开发使用交流电弧等离子体用于气溶生成的AESI源.
- 将AESI源与质量分析仪集成,用于分析物电离和检测.
- 对AESI-MS与ESI-MS进行对电离效率,光谱简单性和盐分耐受性的比较分析.
- 应用AESI-MS用于复杂生物矩阵中氨基酸的定量分析.
主要成果:
- 通过弧形等离子体,AESI-MS有效地产生带电微滴,使分析物的软电离成为可能.
- 该技术结合了APCI和ESI的机制,促进了极性广泛的化合物的电离.
- 与ESI相比,AESI-MS显示了简化的质谱,高盐耐受性和更高的电离效率.
- 使用AESI-MS.实现了复杂矩阵中氨基酸的可靠定量分析.
结论:
- AESI-MS是一种多功能和高效的软电离技术,与传统的ESI相比具有显著的优势.
- 该方法扩大了微滴生成方法,并为新型电子喷雾电离技术提供了强大的框架.
- 在复杂样本中直接分析氨基酸和其他具有挑战性的分析物方面,AESI-MS显示出很大的前景.
更多相关视频
相关概念视频
Electrospray Ionization (ESI) Mass Spectrometry
851
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
851
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
745
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
745
Chemical Ionization (CI) Mass Spectrometry
751
The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
751
Mass Spectrometers
5.5K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.5K
Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle
626
Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
626
Mass Spectrometry: Overview
5.2K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
5.2K


