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相关概念视频

Abnormal Proliferation02:23

Abnormal Proliferation

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Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
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Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
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Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
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The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
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Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
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Updated: Jun 29, 2025

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells
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USP7与SET上蛋白相互作用并使其不稳定.

Jianyuan Chen1, Zishan Jiao1, Yajing Liu1

  • 1State Key Laboratory of Common Mechanism Research for Major Diseases & Department of Medical Genetics, Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, China.

Biochemical and biophysical research communications
|March 31, 2024
PubMed
概括
此摘要是机器生成的。

这项研究确定了泛素特异性蛋白酶7 (USP7) 作为蛋白SE转位 (SET) 的调节者. USP7 破坏了 SET 蛋白水平的稳定性,为癌症治疗提供了一个新的目标.

关键词:
蛋白质与蛋白质的相互作用设置 设置 设置稳定化 稳定化 稳定化两者并排的亲和力净化.USP7 USP7 美国

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相关实验视频

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科学领域:

  • 在瘤学瘤学.
  • 分子生物学分子生物学
  • 生物化学 生化学

背景情况:

  • 上蛋白SE转位 (SET) 在各种癌症中过度表达,与患者预后不佳相关.
  • 准SET是一种有前途的癌症干预策略,但其监管机制尚不清楚.
  • 了解SET监管对于开发有效的癌症疗法至关重要.

研究的目的:

  • 为了识别调节蛋白SET的蛋白质.
  • 为了研究在SET调节中泛素特异性蛋白酶7 (USP7) 的作用.
  • 阐明USP7和SET之间的相互作用.

主要方法:

  • 串联亲和性净化质谱法 (TAP-MS) 用于识别相互作用的蛋白质.
  • 共同免疫沉和西部涂抹以确认蛋白质复合体的形成.
  • USP7 敲击实验,以评估对SET水平的影响.

主要成果:

  • 在癌细胞中,USP7与SET形成稳定的复合体.
  • SET的酸性域与USP7结合;USP7的催化和UBL域是结合所需的.
  • USP7 knockdown 增加了 SET 蛋白质水平而不会影响 SET mRNA,这表明了转录后调节.
  • USP7被确定为第一个与SET.结合的二维基因酶 (DUB).

结论:

  • USP7 破坏了对coprotein SET 的稳定,可能是通过间接机制.
  • USP7在控制SET蛋白的稳定性方面发挥了新的监管作用.
  • 这种相互作用为涉及SET过度表达的癌症提供了潜在的新治疗标.