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相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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相关实验视频

Updated: Jun 27, 2025

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
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Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

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构建一个超高分辨率的光水晶显微镜.

Mart G F Last1, Lenard M Voortman1, Thomas H Sharp2

  • 1Department of Cell and Chemical Biology, Leiden University Medical Centre, Leiden, The Netherlands.

Methods in cell biology
|May 5, 2024
PubMed
概括
此摘要是机器生成的。

我们开发了"低温显微镜",这是一种用于在低温电子显微镜样本上进行超分辨率光显微镜的新型显微镜. 这一创新使得生物结构的高分辨率成像能够在既定的工作流程内进行精确的标记.

关键词:
相对光和电子显微镜的相对光和电子显微镜.显微镜的建造方式超级分辨率的超级分辨率化冷的过程中

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Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
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相关实验视频

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Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

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科学领域:

  • 生物物理学的生物物理.
  • 细胞生物学 细胞生物学
  • 显微镜的使用方法

背景情况:

  • 相关的超分辨率光显微镜 (SMLM) 和冷电子显微镜 (cryo-EM) 提供高标记特异性和分辨率.
  • 整合这些技术需要专门的硬件,能够用超高分辨率光成像冷样本.

研究的目的:

  • 设计和描述一种新型显微镜,即"冷镜",用于冷EM样本的SMLM.
  • 将SMLM集成到现有的冷EM工作流程中.

主要方法:

  • "冷镜"显微镜的设计和制造.
  • 在EM网格上的U2OS细胞中使用SMLM对光标记的维门进行成像.
  • 显微镜的完整设计的3D建模.

主要成果:

  • 展示冷镜对生物样品高分辨率成像的能力.
  • 在U2OS细胞内成功成像光标记的维丁.
  • 为显微镜的设计提供详细的3D模型.

结论:

  • 低温镜通过将高标记特异性和分辨率相结合,使相关的SMLM和冷EM成像成为可能.
  • 开发的显微镜可以无地集成到已建立的冷EM工作流程中.
  • 详细的设计信息为冷镜提供.