Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Volume-sensitive anion conductivity in a human prostatic carcinoma cell line].

Ia M Shuba1, R N Skryma, N B Prevars'ka

  • 1A. A. Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine.

Fiziolohichnyi Zhurnal (Kiev, Ukraine : 1994)
|September 4, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Changes of neuromuscular transmission in smooth muscles of rats bladder with experimental diabetes].

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)·2014
Same author

[Urothelium-dependent modulation of urinary bladder smooth muscle contractions by menthol].

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)·2012
Same author

[Comparative effects of menthol and icilin on the induced contraction of the smooth muscles of the vas deferens of normal and castrated rats].

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)·2011
Same author

[Changes in calcium-dependent potassium channels of isolated smooth muscle cells of the bladder in rats with experimental diabetes].

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)·2011
Same author

[Effects of N-stearoyl- and N-oleoylethanolamine on cardiac voltage-dependent sodium channels].

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)·2011
Same author

[Modulation of the smooth muscle contractions of the rat vas deferens by TRPM8 channel agonist menthol].

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)·2010
Same journal

THE PECULIARITIES OF ARGINASE PATHWAY OF L-ARGININE IN SPERMATOZOA IN MEN WITH DIFFERENT FORMS OF PATHOSPERMIA.

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)·2018
Same journal

THE EFFECT OF GADOLINIUM ORTHOVANADATE NANOPARTICLES BY NEONATAL INDUCED REPRODUCTIVE DISEASE IN MALE RATS.

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)·2018
Same journal

STATUS OF GLUTATHIONE-DEPENDENT LINK OF BLOOD ANTIOXIDANT SYSTEM UNDER APPLICATION OF FENUGREEK SEED POWDER IN ANIMALS WITH EXPERIMENTAL OBESITY.

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)·2018
Same journal

COMPARISON OF SPECTROSCOPIC PROPERTIES OF INTRAOCULAR FLUID IN PATIENTS WITH CATARACT AND PRIMARY OPEN-ANGLE GLAUCOMA.

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)·2018
Same journal

EFFECT OF HYDROGEN SULPHIDE DONOR ON INTRAOCULAR PRESSURE IN RATS.

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)·2018
Same journal

Flavonoid quercetin reduces gliosis after repetitive mild traumatic brain injury in mice.

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)·2018
See all related articles

For the first time, anion currents activated by cell swelling were identified in human prostate cancer cells (LNCaP) exposed to hypotonic solutions. These currents, crucial for cell volume regulation, were reversible and showed non-absolute chloride ion selectivity.

Area of Science:

  • Physiology
  • Cell Biology
  • Oncology

Context:

  • Human prostate carcinoma cell line LNCaP
  • Cell swelling induced by hypotonic solutions
  • Membrane ion transport mechanisms

Purpose:

  • To identify and characterize hypotonicity-evoked membrane anion currents in LNCaP cells
  • To investigate the kinetics and reversibility of these currents
  • To assess the ion selectivity of the involved channels

Summary:

  • First identification of membrane anion current activated by cell swelling in LNCaP cells upon hypotonic shock.
  • Current development reached maximum within 5-10 minutes and was reversible upon return to isotonic conditions.
  • Cell volume-sensitive channels demonstrated non-absolute selectivity for chloride ions.

Related Experiment Videos

Impact:

  • Provides novel insights into ion transport and cell volume regulation in prostate cancer.
  • Potential implications for understanding LNCaP cell behavior and developing targeted therapies.
  • Contributes to the broader knowledge of volume-regulated anion channels (VRACs) in cancer biology.