Rapid identification of Mg-chelated chlorins by on-line high performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry
Related Experiment Video
Updated: Oct 16, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
L Verzegnassi1, C Riffé-Chalard, F O Gülaçar
1Laboratoire de Spectrométrie de Masse, Université de Genève, Geneva, Switzerland.
Abstract:
On-line high performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry (HPLC/APCI-MS) has been applied to the identification of a number of tetrapyrrolic pigments as well as several magnesium-free analogs. Mass spectra, acquired both in positive (+) and negative (-) ionization mode, allow not only the determination of the structural features of the pigments, but also a very easy differentiation of the Mg-chelated pigments from the free bases. In the positive ionization mode, all pigments show mainly a [M + H]+ ion and a [M + H - C20H38]+ fragment ion corresponding to the loss of the phytyl chain via a hydrogen rearrangement. In the negative ionization mode, on the other hand, although all pigments give an abundant molecular anion [M]-*, only the Mg-chelated chlorin spectra show a prominent fragment [M - C20H39]- produced by a formal loss of the phytyl radical.
More Related Videos
07:45Technical Approach for Structural Analysis of an Unknown Compound in Huoxiang Zhengqi Oral Liquid based on Linear Ion Trap Mass Spectrometry
Published on: April 3, 2026
09:04Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow
Published on: April 18, 2019
Related Concept Videos
High-Performance Liquid Chromatography: Types of Detectors
Gas Chromatography: Types of Detectors-II
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Chemical Ionization (CI) Mass Spectrometry
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Rapid Identification of Pathogens