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A plethysmometric method for gastric compliance studies in anesthetized rats
J R Graca1, P R Leal, F A Gondim
1Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, C.P. 3157, 60.430-270, Fortaleza, Brazil.
Journal of Pharmacological and Toxicological Methods
|November 25, 2000
Summary
This study introduces a novel plethysmography method to measure gastric volume and compliance in rats. Yohimbine increased gastric volume, while bethanechol decreased it, demonstrating the method's utility.
Area of Science:
- Physiology
- Gastroenterology
- Medical Devices
Background:
- Assessing gastric volume and compliance is crucial for understanding gastrointestinal function.
- Existing methods may have limitations in accuracy or invasiveness.
Purpose of the Study:
- To develop and validate a new plethysmography-based method for measuring gastric volume and compliance in anesthetized rats.
- To investigate the effects of yohimbine and bethanechol on gastric volume using this new method.
Main Methods:
- A plethysmometer system with a gastric balloon catheter was utilized in Wistar rats.
- Gastric volume changes were recorded, and gastric compliance was calculated.
- Hemodynamic parameters (mean arterial pressure, heart rate) were monitored.
- Animals received intravenous injections of yohimbine or bethanechol.
Main Results:
- The plethysmography method showed high accuracy (r² = 0.99) in calibrating volume changes.
- Yohimbine (1 mg/kg) significantly increased gastric volume by 50%.
- Bethanechol (3 μg/kg) significantly decreased gastric volume by 35%.
- Both drugs caused transient hypotension and bradycardia.
Conclusions:
- A reliable and direct method for assessing gastric compliance in rats using plethysmography has been established.
- Pharmacological agents like yohimbine and bethanechol can modulate gastric volume.
- This technique offers a valuable tool for gastrointestinal research.