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Cell type-dependent collagen-type recognition by cell receptors.
L A Sadofiev1, N S Nikolaenko, N V Kalmykova
1Institute of Cytology RAS, Tikhoretsky pr. 4, 194064, St Petersburg, Russia.
Cell Biology International
|July 13, 2001
Summary
Cells recognize specific collagen types through membrane proteins, with the alpha(2) integrin chain playing a key role in distinguishing between collagen types I and III. This recognition is dependent on the cell type and its origin.
Area of Science:
- Biochemistry
- Cell Biology
- Molecular Biology
Background:
- Collagen-type recognition is crucial for connective tissue cell function.
- Specific cell surface proteins mediate interactions with different collagen types.
Purpose of the Study:
- To identify and characterize cell surface proteins involved in collagen type recognition.
- To investigate the role of integrins in discriminating between collagen types I, III, and IV.
Main Methods:
- Affinity chromatography using collagen types I and III.
- Immunofluorescence using polyclonal antibodies against identified proteins.
- Western blotting with monoclonal antibodies against integrin alpha(2) chain.
Main Results:
- Three collagen-binding proteins (250, 170, and 140 kDa) were identified.
- These proteins distinguished between collagen types I and III, but not III and IV.
- The 140 kDa protein was identified as the alpha(2) integrin chain, localized to the cell membrane.
- Integrin alpha(2) chain-containing receptors mediate collagen type I and III discrimination.
Conclusions:
- Collagen-type recognition is a characteristic feature of connective tissue cells.
- The alpha(2) integrin chain is involved in discriminating between collagen types I and III.
- Collagen recognition by integrins is cell-type dependent.