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Updated: Aug 13, 2026

Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking
Published on: March 16, 2017
Microtubular proteins and concanavalin A receptors
Abstract:
The inherent topographical distribution of Con A binding sites (CABS) is disperse or random in all cell types studied using hemocyanin to mark CABS in surface replicas. In virally transformed cells, the addition of Con A leads to the formation of clusters (CABS). A role for microtubules is suggested in this process since colchicine treatment of transformed cells and Con A addition lead to the aggregation of Con A into a "cap". During phagocytosis CABS are selectively removed from the surface. This selective movement is abolished by drugs that disrupt microtubules. Binding of Con A or RCA to intact cells at 37 degrees C leads to the removal of their receptors from the surface, presumably by "micropinocytosis".
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