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Electrospray mass spectrometry for fumonisin detection and method validation
Steven M Musser1, Robert M Eppley, Mary W Trucksess
1Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Washington, DC 20204, USA.
Fumonisins, toxic compounds in corn, require accurate detection methods. Liquid chromatography, ELISA, and LC/MS are key analytical techniques for quantifying these mycotoxins in food.
Area of Science:
- Food safety and toxicology
- Analytical chemistry
Background:
- Fumonisins are mycotoxins commonly found in corn and corn-based foods.
- They possess a characteristic 19-20 carbon aminopolyhydroxy-alkyl chain structure.
- Fumonisins are associated with various toxicities in animals.
Purpose of the Study:
- To review and compare analytical methods for fumonisin detection.
- To highlight the importance of accurate fumonisin quantification in food products.
Main Methods:
- Liquid chromatography (LC) with fluorescence detection.
- Enzyme-linked immunosorbent assay (ELISA).
- Liquid chromatography/mass spectrometry (LC/MS).
Main Results:
- LC and ELISA provide quantitative measurements of fumonisins.
- LC/MS offers both quantitative analysis and identity confirmation.
- These methods are crucial for monitoring fumonisin levels in food.
Conclusions:
- Accurate analytical methods are essential for managing fumonisin risks in food.
- A range of techniques exist, each with specific advantages for fumonisin detection.
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