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[Characterization of nitric oxide synthase in the chicken cochlea using electrochemical method]
1Department of Otolaryngology, Union Hospital, Tongji Medical University, Wuhan 430022.
Objective:
Our investigation was aimed to explore whether certain agonists can induce NO release from neurons of chick cochlea in vitro to characterize NOS isoform in the cochlea.
Method:
The experiments were performed using CuPtCl6 chemically modified carbon fiber microelectrode under current recording in terms of NO redox activity.
Result:
In our previous study it has been shown that solutions containing L-arg, ATP, ACh with Ca2+ can mediate NO release from neurons of chick cochlea, respectively. The present study examined visual current changes induced by other stimuli to cochlear neurons. The solution containing glutamate with Ca2+ could exerted a positive effects and L-arg, ATP, ACh, glutamate without Ca2+ and bradykimin with Ca2+ or without Ca2+ can not induce NO release from the neurons, respectively.
Conclusion:
Based on these results, the NOS isofrom in the chick cochlear neurons is the neuronal isofrom (ncNOS).