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Antioxidant effect of flavonoids on DCF production in HL-60 cells
Satoshi Takamatsu1, Ahmed M Galal, Samir A Ross
1National Center for Natural Products Research and Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, Mississippi 38677, USA. stakamat@olemiss.edu
Phytotherapy Research : PTR
|September 19, 2003
Abstract:
A fluorometric microplate assay was performed for the detection of respiratory burst activity in a human leukaemia cell line HL-60 by assessing oxidation of 2',7'-dichloro fluorescin diacetate (DCFH-DA). This method is based on the detection of DCFH oxidation due to the presence of hydrogen peroxide. In the present study, the antioxidant activity of a number of structurally related flavonoids of plant origin and some of their microbial transformation products (1-18) were evaluated.