Quantitative PCR analysis of FosB mRNA expression after short duration oxygen and light stress
Scott F Geller1, Jonathan Stone
1School of Optometry, University of California, Berkeley, CA 94720-2020, USA. sgeller@socrates.berkeley.edu
Abstract:
Quantitative polymerase chain reaction (QPCR) was used to examine changes in FosB mRNA expression in models of oxygen and light stress to the retina. C57BL/6 mice or Sprague-Dawley (SD) albino rats were subjected to several experimental paradigms: short-term light or oxygen stress, extended hyperoxia (75% oxygen), or a model of oxygen-induced retinopathy (OIR). Control animals were subjected to room air and 5 lux cyclic light. FosB expression dramatically increases in response to light stress as well as in a model of OIR, but not in response to sustained 75% oxygen. These data suggest that both hypoxia and light stress induce expression of FosB in the retina.
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