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Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils
Published on: November 16, 2015
[Expression of human beta-defensin in palatine tonsil]
Chengshuo Wang1, Zhen Dong, Zhanquan Yang
1Department of Otolaryngology-Head and Neck Surgery, China-Japan Union Hospital of Jilin, University, Changchun, 130031, China.
Objective:
To examine the expression of human beta-defensin-1 (hBD-1) and human beta-defensin-2 (hBD-2) in palatine tonsil of normal subjects and patients with chronic tonsillitis, and to investigate the role of palatine tonsil in the innate immunity system.
Method:
Eighteen specimens of hyperplastic tonsils (n = 8) and chronic tonsillitis(n = 10) were examined using reverse transcriptase-polymerase chain reaction (RT-PCR).
Result:
beta-defensin 1 mRNA was expressed in all tissue specimens, at levels that did not differ significantly (P > 0.05). beta-defensin 2 mRNA was detected in the specimens of chronic tonsillitis, but almost not in the normal subjects. Its expression level was significantly higher in chronic inflamed tonsils than in normal controls(P < 0.05).
Conclusion:
These results suggest that beta-defensin of the palatine tonsil may play an important role in innate defense against microorganisms.
