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POSaM: a fast, flexible, open-source, inkjet oligonucleotide synthesizer and microarrayer.
Christopher Lausted1, Timothy Dahl, Charles Warren
1The Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA. clausted@systemsbiology.org
Genome Biology
|August 4, 2004
Summary
Researchers developed an open-source inkjet arrayer for creating DNA arrays. This flexible technology rapidly synthesizes unique oligonucleotide sequence arrays, complementing existing microarray methods.
Area of Science:
- Molecular Biology
- Genomics
- Biotechnology
Background:
- DNA arrays are essential tools in molecular biology research.
- The adoption of DNA arrays was accelerated by the Stanford University's release of pin-spotting microarrayer plans.
- Inkjet microarraying offers a flexible alternative for synthesizing custom oligonucleotide arrays.
Purpose of the Study:
- To describe an open-source inkjet arrayer.
- To demonstrate its capability in rapidly producing high-density arrays.
- To provide a complementary technique to existing pin-spotting microarrayers.
Main Methods:
- Development of an open-source inkjet microarrayer.
- Utilizing inkjet technology for de novo synthesis of oligonucleotide sequences.
- Fabrication of arrays with 9,800 unique features.
Main Results:
- Successful rapid production of unique 9,800-feature DNA arrays.
- Demonstration of inkjet microarraying as a viable and flexible technique.
- Creation of an open-source platform for broader laboratory access.
Conclusions:
- The developed open-source inkjet arrayer is a valuable tool for molecular biology.
- Inkjet microarraying provides a flexible and efficient method for generating custom oligonucleotide arrays.
- This technology complements existing microarray approaches and promotes accessibility in research.