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Updated: Aug 22, 2026
Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
Published on: October 8, 2013
[Ciliogenesis in human nasal epithelial cells cultured at the air-liquid interface]
Yongqing Guo1, Xiaodong Zhao, Zhanquan Yang
1Department of Otolaryngology, First Clinical Academy of Medical College of Xiamen University, Xiamen, 361004, China.
Objective:
In the present study, our aim was to develop a method to quantitative cell differentiation in cultures of human nasal epithelium (HNE) cells.
Method:
HNE cells were cultured on collagengel-coated membranes at an air-liquid interface (ALI) in hormone-and growth factor-supplemented medium. The percentage of the culture surface covered with ciliated cells was estimated using scanning electron microscope and image analysis.
Result:
In present study, if an ALI was not established and the cells were maintained in the submerged state, ciliated cell differentiation was pool, the average ciliated surface area was 0.3% when cultures were submerged for fourteen days. When HNE cells cultures in ALI, average ciliated surface area was 8.6%.
Conclusion:
Our study demonstrated increased ciliogenesis in ALI.
