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Updated: Aug 11, 2026
Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model
Published on: May 21, 2013
[The study of producing allogenic-tissue engineering cartilage]
Wei Lü1, Lianshan Zhang, Zhe Cai
1Department of Otorhinolaryngology, Peking Union Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China.
Objective:
To explore the feasibility of producing neocartilage tissue by using allogenic chondrocytes as seeding cells, artificial absorbable scaffold-polylactic acid (PLA) as matrices.
Method:
The cartilage tissue was obtained from the four limbs of neoborn New Zealand rabbits (born 3-5 days), digested by II collagenase, proliferated in vitro, then seeded the chondrocytes on PLA scaffolds. The chondrocyte-PLA complex were cultured 7-10 days in vitro, then implanted in dorsal subcutaneous of adult New Zealand rabbits. Specimens harvested 3 weeks, 5 weeks and 7 weeks after implantation respectively and subjected to histologic analysis.
Result:
Chondrocyte-PLA complex implanted in adult new Zealand rabbits for 7 weeks, produced neo-cartilage as proved by histologic analysis, along with time extending, chondrocyte matrice secretion increased, invading inflammatory cell decreased.
Conclusion:
Using allogenic chondrocytes as seeding cells and PLA scaffold, it is successful to generate allogenic tissue-engineering cartilage in animals that have inherent immunity.
