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Updated: Aug 17, 2026
Induction of Ocular Surface Inflammation and Collection of Involved Tissues
Published on: August 4, 2022
[The expression of cytokines in experimental otitis media with effusion]
Rong Sun1, Suling Hong, Xinye Tang
1Department of Otorhinolaryngology, the First Hospital, Chongqing Medical University, Chongqing, 400016, China. sunr2002@eyou.com
Objective:
To explore the relationship between type I allergic reaction and OME and the expression of Th1, Th2 cytokines in the middle ear mucosa in OME.
Method:
BALB/C mice were randomly divided into experimental group (Exp), control group I (Con1) and control group II (Con2), BALB/C mice were sensitized to ovalbumin (OVA) and challenged transtympanically. The thickness of mucosa, the infiltration of eosinophils were detected by hematotoxylin-eosin staining (HE staining), the expression of IL-4, IL-5, IFN-gamma and IL-12 were examined by immunohistochemistry.
Result:
In experimental group, the expression of IL-4, IL-5, the thicknesss of mucosa and the numbers of eosinophils were significantly higher than those of Con1, Con2. But the expression of IFN-gamma, IL-12 were both significantly low. The concentration of IL-5 were positively correlated with the number of eosinophils in mucosa.
Conclusion:
This study demonstrates that type I allergic reaction, Th2 cytokines have an important effect on OME.
