[Identification of pentazocine, piritramide and methadone in blood using thin layer chromatography]

Pentazocine, piritramide and methadone, when extracted from blood or plasma with ether at pH ca. 12, were separated by TLC method on alumina or silica gel by ascending technique on 5 x 20 cm and 2.5 x 7.5 cm plates and also by horizontal development, using suitable mobile phases. The substances were identified by reaction with potassium iodoplatinate or 20% Na2CO3 solution (up to the amount 2 micrograms pentazocine, 0.5 microgram piritramide and 1.5 micrograms methadone.