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Published on: January 7, 2020
Macrophage behavior on multilayered DNA-coatings in vitro
J J J P Van den Beucken1, X F Walboomers, M R J Vos
1Department of Periodontology and Biomaterials, Radboud University Nijmegen Medical Center, PO Box 9101, Nijmegen 6500 HB, the Netherlands.
Journal of Biomedical Materials Research. Part A
|October 13, 2006
Summary
Novel DNA-based biomaterial coatings show potential for reduced inflammation. Macrophages cultured on these coatings exhibited reduced tumor necrosis factor-alpha (TNF-alpha) secretion, suggesting improved biocompatibility for implants.
Area of Science:
- Biomaterials Science
- Immunology
- Cell Biology
Background:
- The inflammatory response to biomaterials is critical for implant success.
- Macrophages are key immune cells influencing biomaterial interactions and signaling molecule production.
- Understanding cellular behavior on new biomaterial coatings is essential for predicting clinical outcomes.
Purpose of the Study:
- To evaluate protein adsorption on poly-D-lysine (PDL) or poly(allylamine hydrochloride) (PAH) and DNA multilayered biomaterial coatings.
- To assess the in vitro behavior of macrophages, including attachment, viability, and morphology, on these novel coatings.
- To compare the effects of DNA-coatings with negative (uncoated glass) and positive (LPS-stimulated uncoated glass) controls.
Main Methods:
- Fabrication of multilayered DNA-coatings using PDL or PAH.
- Quantification of gross protein adsorption on coated and uncoated surfaces.
- In vitro culture of two macrophage types on the biomaterial coatings.
- Assessment of macrophage attachment, viability, morphology, and cytokine secretion (TNF-alpha, IL-1beta, IL-10, TGF-beta1).
Main Results:
- Multilayered DNA-coatings did not significantly alter gross protein adsorption compared to controls.
- Macrophage attachment, viability, and morphology on DNA-coatings were comparable to noncoated glass.
- Macrophages cultured on DNA-coatings demonstrated decreased secretion of the proinflammatory cytokine TNF-alpha, with no significant changes in IL-1beta, IL-10, or TGF-beta1.
Conclusions:
- Novel multilayered DNA-coatings do not adversely affect protein adsorption or basic macrophage behavior in vitro.
- A reduction in TNF-alpha secretion by macrophages on DNA-coatings suggests a potentially modulated inflammatory response.
- Further in vivo studies are necessary to determine the clinical implications of these in vitro findings for implant-associated inflammation and wound healing.
