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Updated: Jul 18, 2026

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
Published on: February 17, 2023
Design of sensing ribonucleopeptides for small ligands
Masaki Hagihara1, Tetsuya Hasegawa, Yukiko Tanabe
1Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan.
Abstract:
Here we report a simple method to convert synthetic receptors to fluorescent sensors. An RNA-peptide complex (ribonucleopeptide) with a known three-dimensional structure is used as a framework of the receptor. Artificial ribonucleopeptide sensors were created with a combination of in vitro selection method and successive modification of the peptide with a fluorophore. A ribonucleopeptide complex of the fluorophore labeled peptide showed a remarkable fluorescence emission change upon binding cognate ligands.
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