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Real-time monitoring of restriction endonuclease activity using molecular beacon

Changbei Ma1, Zhiwen Tang, Kemin Wang

  • 1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Engineering Research Center for Bio-Nanotechnology of Hunan Province, Hunan University, Changsha 410082, People's Republic of China.

Analytical Biochemistry
|February 23, 2007
PubMed
Abstract

No abstract available in PubMed .

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Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
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