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Updated: Jul 13, 2026
10:38
Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli
Published on: November 30, 2018
How a small DNA virus uses dsRNA but not RNAi to regulate its life cycle
R Gu1, Z Zhang, G G Carmichael
1Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA.
Cold Spring Harbor Symposia on Quantitative Biology
|March 27, 2007
Summary
Mouse polyomavirus RNA editing regulates gene expression. Multigenomic RNAs are produced via polyadenylation, downregulating early gene expression through RNA editing.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Mouse polyomavirus has a circular DNA genome with early and late genes on opposite strands.
- Early gene expression dominates initially, followed by late gene expression after viral DNA replication.
Purpose of the Study:
- To elucidate the regulatory mechanisms of mouse polyomavirus gene expression.
- To investigate the role of RNA editing in controlling viral transcription and replication.
Main Methods:
- Analysis of viral RNA transcripts at different infection stages.
- Investigating polyadenylation efficiency and RNA editing of viral RNAs.
- Developing a model for multigenomic RNA production and its regulatory impact.
Main Results:
- Late primary transcripts are inefficiently polyadenylated, forming multigenomic RNAs.
- These multigenomic RNAs downregulate early gene expression via RNA editing.
- RNA editing of poly(A) signals, directed by overlapping transcripts, controls multigenomic RNA production.
Conclusions:
- RNA editing is a key mechanism for regulating mouse polyomavirus gene expression.
- The interplay between polyadenylation and RNA editing controls viral gene transcription.
- A novel model explains multigenomic RNA production and its regulatory role in viral infection.
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