Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Layered peptide array for multiplex immunohistochemistry.

Gallya Gannot1, Michael A Tangrea, Heidi S Erickson

  • 1Pathogenetics Unit, Advanced Technology Center, Laboratory of Pathology and Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892-4605, USA.

The Journal of Molecular Diagnostics : JMD
|June 27, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unraveling Links between Chronic Inflammation and Long COVID: Workshop Report.

Journal of immunology (Baltimore, Md. : 1950)·2024
Same author

Adhesion Characterization and Enhancement between Polyimide-Silica Composite and Nodulated Copper for Applications in Next-Generation Microelectronics.

ACS applied materials & interfaces·2024
Same author

Environmental epidemiology and risk assessment: Exploring a path to increased confidence in public health decision-making.

Global epidemiology·2023
Same author

Effect of Antigen Retrieval on Genomic DNA From Immunodissected Samples.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2022
Same author

Microdissection Methods Utilizing Single-Cell Subtype Analysis and the Impact on Precision Medicine.

Methods in molecular biology (Clifton, N.J.)·2022
Same author

Metabolic Regulation of Inflammation and Its Resolution: Current Status, Clinical Needs, Challenges, and Opportunities.

Journal of immunology (Baltimore, Md. : 1950)·2021
Same journal

Epigenetic CD4+ T-Cell Quantification from Dried Blood Spots Using a qPCR-Based Assay.

The Journal of molecular diagnostics : JMD·2026
Same journal

Segmental Copy Number Variant Detection Using an Amplicon-based NGS Panel for Integrated Glioma Classification.

The Journal of molecular diagnostics : JMD·2026
Same journal

Clinical Validation of the Roche cobas and cobas 4800 Human Papillomavirus Tests on Self-Collected Vaginal Dry Swabs versus Practitioner-Collected Cervical Specimens Using the VALHUDES Protocol.

The Journal of molecular diagnostics : JMD·2026
Same journal

Long-Read Nanopore Sequencing Enhances BRCA1/2 Variant Detection Compared with Ion Torrent Analysis.

The Journal of molecular diagnostics : JMD·2026
Same journal

Endonuclease-Assisted Selective Exponential Amplification for Ultrasensitive Enrichment and Detection of Low-Abundance Mutant Alleles in Lung Cancer.

The Journal of molecular diagnostics : JMD·2026
Same journal

Validation of NTRK Fusion Detection Using an Ultrarapid, Fully Automated Cartridge-Based PCR Assay.

The Journal of molecular diagnostics : JMD·2026
See all related articles

Indirect layered peptide arrays (iLPA) offer a simple, inexpensive method for multiplex protein measurement in tissue sections. This new technology shows promise for molecular diagnostics and research applications.

Area of Science:

  • Life science
  • Proteomics
  • Molecular diagnostics

Background:

  • Multiplex molecular measurements are crucial for understanding biological systems.
  • Existing methods for analyzing proteins on solid surfaces can be complex or costly.

Purpose of the Study:

  • To describe and evaluate an indirect layered peptide array (iLPA) for protein measurement on tissue sections.
  • To assess the reproducibility, specificity, and potential applications of the iLPA technology.

Main Methods:

  • Development of a prototype indirect layered peptide array (iLPA) system.
  • Examination of iLPA for reproducibility and specificity.
  • Comparison of iLPA with standard immunohistochemistry techniques.

Main Results:

Related Experiment Videos

  • Successful semiquantitative, multiplex proteomic analysis of histological sections was achieved.
  • The iLPA system demonstrated experimental variability of 18%.
  • The iLPA technology proved capable of measuring target antigens on tissue sections.

Conclusions:

  • Indirect layered peptide array (iLPA) technology provides a relatively simple and inexpensive method for molecular measurements from tissue sections.
  • iLPA is a promising tool for multiplex proteomic analysis in research and diagnostics.