Related Experiment Video
Updated: Jul 11, 2026
13C6-Glucose Labeling Associated with LC-MS: Identification of Plant Primary Organs in Secondary Metabolite Synthesis
Published on: March 22, 2024
[Studies on the extraction process of total saponins from Paris polyphylla Smith]
Zhi-Guo Sun1, Lin Zhang, Ling-Jun Li
1College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Objective:
To optimize the extraction process of total saopnins from Paris polyphylla Smith.
Methods:
The single factor test and orthogonal experiment were used to determine the optimum extraction process.
Results:
The optimum extraction process was obtained as follows: the plant materials were extracted with 70% ethanol twice, respectively with 10BV for 2 hours and then with 8BV the solvents for 1.5 hours. The yield of total saponins could be up to 4.24% and the total extraction rate of Paris polyphylla I and Paris polyphylla II was 93.28%.
Conclusion:
The optimum process obtained is steady, reasonable and feasible.
