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High-performance liquid chromatographic determination of phenylacetic acid in human plasma extracted with ethyl
1Institution Universitaire de Psychiatrie, Service de la Recherche Biologique et de Psychopharmacologie Clinique, Chêne-Bourg, Geneva, Switzerland.
Journal of Chromatography
|October 4, 1991
Abstract:
This paper describes a high-performance liquid chromatographic method with ultraviolet detection for measuring plasma phenylacetic acid. This simple and reliable method consists of an acid hydrolysis of conjugated phenylacetic acid before extraction with an organic solvent: washed ethyl acetate saturated in sodium chloride. The recovery of extraction was estimated by internal standardization with phenylpropionic acid, and validated by addition of phenylacetic acid standards. A preliminary application to plasma phenylacetic acid in patients suffering from depression is described.