Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hybridization analysis using oligonucleotide probe arrays.

Robert S Matson1, Jang B Rampal

  • 1Beckman Coulter, Inc., Fullerton, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 7, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ELISA Essentials: Surfaces, Antibodies, Enzymes, and Substrates.

Methods in molecular biology (Clifton, N.J.)·2023
Same author

Antibody Immobilization.

Methods in molecular biology (Clifton, N.J.)·2023
Same author

Interference in ELISA.

Methods in molecular biology (Clifton, N.J.)·2023
Same author

Lateral Flow Microarray-Based ELISA for Cytokines.

Methods in molecular biology (Clifton, N.J.)·2023
Same author

A Unique Multiplex ELISA to Profile Growth Factors and Cytokines in Cerebrospinal Fluid.

Methods in molecular biology (Clifton, N.J.)·2023
Same author

Well-Based Multiplex Food Allergen Colorimetric ELISA.

Methods in molecular biology (Clifton, N.J.)·2023
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

This study details methods for DNA labeling, hybridization, and detection using oligonucleotide probes on plastic substrates. It offers a systematic approach for accurate target DNA discrimination through optimized hybridization and wash stringency.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genetics

Background:

  • Oligonucleotide microarrays are crucial for genetic analysis.
  • Plastic substrates offer advantages for microarray development.
  • Efficient DNA detection and discrimination are essential for molecular diagnostics.

Purpose of the Study:

  • To describe a methodology for labeling, hybridizing, and detecting amplicon target DNA.
  • To present a systematic approach for target DNA discrimination.
  • To optimize hybridization and wash stringency for accurate results.

Main Methods:

  • Labeling of amplicon target DNA.
  • Hybridization of labeled DNA to oligonucleotide probe arrays on plastic substrates.
  • Detection of hybridized DNA.

Related Experiment Videos

  • Application of systematic wash stringency protocols.

    • Main Results:

    • Successful labeling, hybridization, and detection of target DNA were achieved.
    • The methodology allows for effective discrimination of target DNA sequences.
    • Wash stringency is a key factor in achieving specific hybridization.

    Conclusions:

    • The described methodology provides a robust platform for DNA analysis on plastic microarrays.
    • Systematic optimization of hybridization and wash stringency enhances target discrimination.
    • This approach has implications for various molecular biology applications, including diagnostics.