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Updated: Jul 6, 2026
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Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange
Published on: April 27, 2017
Developing reagents and conditions to induce mesoderm subsets from ES cells.
1Department of Pediatrics, Indiana University School of Medicine, 1044 West Walnut Street, R4-402e, Indianapolis, IN 46202, USA. myoder@iupui.edu
Cell Stem Cell
|March 29, 2008
Summary
Researchers used selectable markers to track gene expression during embryonic stem cell differentiation. This method helps understand how specific mesoderm cell types develop from stem cells.
Area of Science:
- Developmental biology
- Stem cell research
- Cellular differentiation
Background:
- Embryonic stem (ES) cell differentiation provides a model for studying early developmental processes.
- Understanding lineage-specific gene expression is crucial for dissecting developmental pathways.
Purpose of the Study:
- To investigate the induction of mesoderm subsets during ES cell differentiation.
- To utilize selectable markers for detecting lineage-specific gene expression.
Main Methods:
- Employing selectable markers to monitor gene expression in differentiating ES cells.
- Analyzing the induction of specific mesodermal lineages.
Main Results:
- Successful detection of lineage-specific gene expression using selectable markers.
- Dissection of the induction process for distinct mesoderm subsets.
Conclusions:
- Selectable markers are effective tools for studying ES cell differentiation and lineage commitment.
- The study provides insights into the molecular mechanisms governing mesoderm development from stem cells.
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00:58Embryonic Stem Cells
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
00:57Embryonic Stem Cells
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...