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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Updated: Jul 6, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Integrated Raman- and angular-scattering microscopy.

Zachary J Smith1, Andrew J Berger

  • 1The Institute of Optics, University of Rochester, Rochester, NY 14627, USA.

Optics Letters
|April 3, 2008
PubMed
Summary
This summary is machine-generated.

This study introduces a novel microscopy system for simultaneous Raman spectroscopy and angle-resolved elastic scattering. This innovation enables precise single-sphere diameter measurement and high-quality Raman data acquisition for potential single-cell analysis.

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Area of Science:

  • Optical microscopy
  • Spectroscopy
  • Nanoscale metrology

Background:

  • Traditional Raman spectroscopy and elastic scattering measurements are often performed separately.
  • Accurate characterization of micro- and nanoparticles is crucial for various scientific disciplines.
  • Generalized Lorenz-Mie theory provides a theoretical framework for analyzing light scattering from spheres.

Purpose of the Study:

  • To develop and validate a microscopy system capable of simultaneous Raman spectra and angle-resolved elastic scattering.
  • To experimentally verify predictions of generalized Lorenz-Mie theory regarding single-sphere backscatter.
  • To demonstrate the system's capability for precise particle sizing and high-quality spectral acquisition.

Main Methods:

  • Construction of a microscopy system utilizing a single focused laser spot (<10 µm).
  • Simultaneous acquisition of Raman spectra and angle-resolved elastic scattering patterns.
  • Analysis of elastic scattering data using generalized Lorenz-Mie theory.

Main Results:

  • The system successfully acquired both Raman spectra and elastic scattering patterns concurrently.
  • Experimental validation of generalized Lorenz-Mie theory's prediction of angular backscatter from single spheres.
  • Demonstrated 3 nm precision in predicting sphere diameters while obtaining high-quality Raman signals.

Conclusions:

  • The developed microscopy system offers a powerful tool for simultaneous optical measurements.
  • The findings validate theoretical predictions and showcase the system's metrological capabilities.
  • The system holds significant potential for applications in single-cell analysis and material characterization.