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Related Experiment Video

Updated: Jul 5, 2026

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay
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Published on: March 1, 2011

Measurement of human interleukin 11.

Frann Bennett1, JoAnn Gianotti1, Abbie Celniker1

  • 1Genetics Institute, Inc, Cambridge, Massachusetts.

Current Protocols in Immunology
|April 25, 2008
PubMed
Summary
This summary is machine-generated.

This study presents improved methods for measuring human and murine interleukin 11 (IL-11) protein levels and biologic activity. A sensitive cell proliferation bioassay offers enhanced detection limits for IL-11.

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06:29

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Published on: March 1, 2011

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08:26

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Area of Science:

  • Biochemistry
  • Immunology
  • Cell Biology

Background:

  • Interleukin 11 (IL-11) is a cytokine with significant roles in various physiological processes.
  • Accurate measurement of IL-11 protein levels and biologic activity is crucial for research and diagnostics.
  • Existing bioassays for IL-11 may lack sensitivity or specificity.

Purpose of the Study:

  • To describe a sensitive Enzyme-Linked Immunosorbent Assay (ELISA) for quantifying human and murine IL-11 protein.
  • To detail an enhanced cell proliferation bioassay for measuring IL-11 biologic activity.
  • To provide a method for ensuring IL-11 specificity in complex cytokine samples.

Main Methods:

  • Development and validation of an ELISA for IL-11 protein quantification.
  • Establishment of a cell proliferation bioassay using the B9-11 cell line, a subline highly responsive to IL-11.
  • Implementation of a neutralizing antibody procedure within the bioassay to confirm IL-11 activity.

Main Results:

  • The new bioassay demonstrates improved sensitivity for IL-11 detection, with a limit of 20 pg/ml.
  • The B9-11 cell line shows superior responsiveness to IL-11 compared to older T10 cell lines.
  • The neutralizing antibody method effectively ensures measurement of specific IL-11 activity.

Conclusions:

  • The described ELISA and cell proliferation bioassay provide robust tools for measuring IL-11.
  • The enhanced bioassay offers superior sensitivity and specificity for IL-11 activity assessment.
  • These methods advance the ability to study IL-11 in biological samples.