Optimization and validation of capillary electrophoretic method for the analysis of amphenicols in poultry tissues

A simple, rapid capillary electrophoretic (CE) method for simultaneous analysis of three amphenicols (chloramphenicol, thiamphenicol and florfenicol) in poultry tissues has been developed and validated. The separation condition were: buffer solution composed with 25 mM sodium tetraborate decahydrate and 10 mM sodium dodecylsulfate, an uncoated fused-silica capillary (57 cm x 75 microm i.d.), voltage 20 kV, and temperature 22 degrees C. The method involved simple deproteinization by acetonitrile and SPE extraction procedure. The analytical method was validated according to the FDA bioanalytical method guidance. The method was linear (r > 0.999) at concentrations ranging from: 0.005 - 1 for chloramphenicol, 0.01 - 1 for thiamphenicol, and 0.025 - 5 microg/g for florfenicol. The precision values were less than 9.8 for intra- and 14.8% for inter-day variability, and accuracies ranged from 92.0 to 106.0% for analyzed amphenicols. The overall recoveries of all antibiotics from tissue samples were above 82.2%. Some new parameters were calculated as limit of decision (CCalpha) and detection capability (CCbeta). The CE method is simple and reliable for simultaneous determination of residues of chloramphenicol, thiamphenicol and florfenicol in muscle with a total run time of less than 7 min.