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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
FRET-based aptamer probe for rapid angiogenin detection.
Wei Li1, Xiaohai Yang, Kemin Wang
1Engineering Center for Biomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Talanta
|July 1, 2008
Summary
A new fluorescence method rapidly detects angiogenin (Ang) using a dual-labeled aptamer probe. This sensitive assay accurately quantifies Ang in serum, showing potential for lung cancer diagnostics.
Area of Science:
- Biochemistry
- Molecular Biology
- Analytical Chemistry
Background:
- Angiogenin (Ang) is a key protein implicated in angiogenesis and various cancers.
- Accurate and sensitive detection of Ang is crucial for early disease diagnosis and monitoring.
- Existing detection methods may lack the required sensitivity or speed for clinical applications.
Purpose of the Study:
- To develop a highly sensitive and rapid detection method for angiogenin (Ang).
- To utilize fluorescence resonance energy transfer (FRET) with an aptamer-based probe for Ang quantification.
- To validate the assay's performance and applicability in biological samples.
Main Methods:
- Construction of a dual-labeled aptamer probe with a high affinity for Ang.
- Labeling the aptamer with a FRET donor (FAM) and acceptor (TMR) at specific termini.
- Monitoring fluorescence intensity changes upon Ang binding to quantify the protein concentration.
- Validation of the assay using standard solutions and human serum samples.
Main Results:
- The dual-labeled aptamer probe exhibited significant fluorescence changes upon specific binding to Ang.
- The developed FRET assay demonstrated high specificity and sensitivity for Ang detection.
- The limit of detection was determined to be 2.0 x 10(-10) mol L(-1) with a linear range of 5.0 x 10(-10) to 4.0 x 10(-8) mol L(-1).
- Ang was successfully detected in serum samples from both healthy individuals and lung cancer patients.
Conclusions:
- A sensitive and rapid FRET-based assay for angiogenin detection has been successfully developed.
- The aptamer-based probe offers a robust platform for quantitative Ang measurement.
- This method holds promise for the early diagnosis and monitoring of diseases associated with Ang, such as lung cancer.
