Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multicyclic D-Stereospecific Hydrolase Dimer With High Sustained Activity.

Angewandte Chemie (International ed. in English)·2026
Same author

TAV2b Peptide Derivatives Underwind and Stabilize Double-Stranded RNA upon Binding.

Journal of the American Chemical Society·2026
Same author

DNA-programmed bispecific peptide assemblies for delivering cytotoxic payload to cells expressing EGFR and MET receptors.

RSC chemical biology·2025
Same author

Stabilizing Proteins by Chemical Cross-Linking: Insights into Conformation, Unfolding, and Aggregation Using Native Ion Mobility Mass Spectrometry.

Analytical chemistry·2025
Same author

Impact of Glycoclustering on Stiffening of MUC5AC Peptides Revealed by High-Efficiency Synthesis.

Angewandte Chemie (International ed. in English)·2025
Same author

Glycan-induced fluorescence enhancement using a molecular rotor-boronic acid conjugate.

Chemical communications (Cambridge, England)·2025
Same journal

A novel convenient method for high bacteriophage titer assay.

Nucleic acids symposium series (2004)·2009
Same journal

Expression behavior of high-pressure-compacted plasmid DNA in mammalian cell.

Nucleic acids symposium series (2004)·2009
Same journal

Role of exposed aromatic residues in substrate-binding of CBM family 5 chitin-binding domain of alkaline chitinase.

Nucleic acids symposium series (2004)·2009
Same journal

Incipient complex formation between AP endonucleases and DNA containing AP site: a vital role of the tryptophan residue.

Nucleic acids symposium series (2004)·2009
Same journal

Physiological role of RsgA in ribosome biosynthesis.

Nucleic acids symposium series (2004)·2009
Same journal

Trans-translation by tmRNA and SmpB.

Nucleic acids symposium series (2004)·2009
See all related articles

Related Experiment Video

Updated: Jul 1, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Recognizing and controlling biomolecules with "Smart" hybridization-based switches.

Oliver Seitz1, Tom N Grossmann, Stefanie Thurley

  • 1Institute of Chemistry, Humboldt University Berlin, Brook-Taylor-Str. 2, D-12489 Berlin, Germany. oliver.seitz@chemie.hu-berlin.de

Nucleic Acids Symposium Series (2004)
|September 9, 2008
PubMed
Summary
This summary is machine-generated.

Researchers explored how DNA duplex formation rules can be used to create novel molecular probes. These probes can detect nucleic acids or link DNA and peptide structure changes, with applications in bioanalysis and synthetic biology.

More Related Videos

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
09:39

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Published on: March 31, 2022

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
07:16

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

Related Experiment Videos

Last Updated: Jul 1, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
09:39

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Published on: March 31, 2022

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
07:16

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

Area of Science:

  • Molecular Biology
  • Bioanalytical Chemistry
  • Synthetic Biology

Background:

  • The rules governing DNA duplex formation are well-established.
  • DNA structure and reactivity changes are key to molecular recognition.
  • Interactions between nucleic acids and peptides are crucial in biological systems.

Purpose of the Study:

  • To explore the use of DNA duplex formation rules in designing molecular probes.
  • To investigate the transduction of nucleic acid structure changes to peptide structure changes, and vice versa.
  • To discuss applications in bioanalytical chemistry and synthetic biology.

Main Methods:

  • Design of probe molecules based on DNA hybridization principles.
  • Development of molecules capable of signal transduction between nucleic acid and peptide structures.
  • Analysis of probe performance and transduction mechanisms.

Main Results:

  • Demonstrated the utility of DNA duplex formation rules for creating responsive molecular probes.
  • Showcased molecules that link changes in DNA structure to peptide structure, and vice versa.
  • Identified potential applications for these molecular systems.

Conclusions:

  • The principles of DNA duplex formation offer a versatile platform for developing advanced molecular tools.
  • Transduction molecules bridge nucleic acid and peptide functionalities, opening new avenues in molecular design.
  • These advancements hold promise for innovations in bioanalytical chemistry and synthetic biology.