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RNA containing pyrrolocytidine base analogs: increased binding affinity and fluorescence that responds to
Alexander S Wahba1, Masad J Damha, Robert H E Hudson
1Department of Chemistry, McGill University, Montreal, QC, Canada.
Abstract:
6-Phenylpyrrolocytidine and 6-methoxymethylene-pyrrolocytidine are base-modified nucleosides with remarkable fluorescence properties. These analogs produce increased binding affinity to both RNA and DNA targets when incorporated into oligoribonucleotides. The fluorescence observed for the single-stranded oligomers is quenched upon duplex formation with either RNA or DNA targets. The fluorescence response depends on the nature of the 6-substituent and the sequence position of the modified nucleoside.
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