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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
Published on: September 19, 2017
Creatinine biosensor based on ammonium ion selective electrode and its application in flow-injection analysis.
Anna Radomska1, Ewa Bodenszac, Stanisław Gła B
1Department of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.
Talanta
|October 31, 2008
Summary
A novel biosensor offers sensitive and stable creatinine detection. This new method, using immobilized creatinine deiminase, provides rapid analysis for clinical and biomedical applications.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Enzyme Technology
Background:
- Accurate creatinine determination is crucial for diagnosing kidney function and monitoring health.
- Existing methods for creatinine measurement can be time-consuming or lack sensitivity.
- Development of rapid, stable, and sensitive biosensors is essential for clinical diagnostics.
Purpose of the Study:
- To develop a highly sensitive, fast-responding, and stable potentiometric biosensor for creatinine determination.
- To immobilize creatinine deiminase onto an ammonium ion-selective electrode for enhanced performance.
- To validate the biosensor's efficacy in analyzing clinical samples under flow injection analysis (FIA) conditions.
Main Methods:
- Development of a potentiometric biosensor utilizing an ammonium ion-selective electrode.
- Chemical immobilization of creatinine deiminase (EC 3.5.4.21) via carbodiimide covalent attachment to a polymeric ion-sensitive membrane.
- Application of the biosensor in a flow injection analysis (FIA) system for sample analysis.
Main Results:
- The biosensor demonstrated high sensitivity and rapid response for creatinine detection.
- Excellent operational and storage stability were observed, retaining over 70% sensitivity after ten weeks.
- The determination range (0.02–20.0 mmol/L) covers clinically relevant creatinine concentrations.
- Successful determination of creatinine in urine, serum, and posthemodialysate samples was achieved.
Conclusions:
- A stable and sensitive potentiometric biosensor for creatinine determination has been successfully developed.
- The biosensor, integrated with FIA, offers a robust platform for rapid clinical and biomedical analysis.
- The developed system provides a valuable tool for efficient creatinine monitoring in various biological samples.
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