Chemiluminescence thrombin aptasensor using high-activity DNAzyme as catalytic label

Area of Science:

• Biotechnology
• Analytical Chemistry
• Biochemistry

Background:

• Thrombin is a key biomarker in various physiological and pathological processes.
• Accurate thrombin detection is crucial for diagnosing and monitoring diseases.
• Existing detection methods often lack sufficient sensitivity and efficiency.

Purpose of the Study:

• To develop an ultrasensitive chemiluminescence aptasensor for thrombin detection.
• To utilize a high-activity DNAzyme as a catalytic label for enhanced signal amplification.
• To improve the sensitivity of thrombin detection compared to previous analytical platforms.

Main Methods:

• Design and synthesis of a high-activity DNAzyme catalytic label.
• Development of a chemiluminescence-based aptasensor platform.
• Conjugation of the DNAzyme label to aptamers for specific thrombin recognition.
• Optimization of reaction conditions for maximal chemiluminescence signal.

Main Results:

• The developed aptasensor demonstrated ultrasensitive detection of thrombin.
• A significant improvement in detection sensitivity, ranging from 10- to 100-fold, was achieved.
• The aptasensor exhibited high specificity and stability in detection.

Conclusions:

• The novel DNAzyme-aptasensor platform offers a highly sensitive and efficient method for thrombin detection.
• This approach represents a significant advancement in chemiluminescence-based biosensing.
• The developed aptasensor holds potential for clinical diagnostics and biomedical research.