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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
Multifunctional G-quadruplex aptamers and their application to protein detection.
Tao Li1, Lili Shi, Erkang Wang
1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China.
Chemistry (Weinheim an Der Bergstrasse, Germany)
|December 5, 2008
Summary
Two G-quadruplex aptamers, AGRO100 and T30695, show high hemin-binding affinity and peroxidase-like activity. These multifunctional aptamers enable sensitive detection of proteins like nucleolin, expanding their biochemical applications.
Area of Science:
- Biochemistry
- Molecular Biology
- Analytical Chemistry
Background:
- G-quadruplex aptamers are nucleic acid sequences with unique structures.
- Aptamers can bind to various targets, including proteins.
- Developing novel aptamer applications is crucial for biochemical assays.
Purpose of the Study:
- To identify and characterize multifunctional G-quadruplex aptamers.
- To evaluate the hemin-binding affinity and peroxidase-like activity of these aptamers.
- To demonstrate the application of these aptamers in sensitive protein detection.
Main Methods:
- Selection and characterization of G-quadruplex aptamers AGRO100 and T30695.
- Assessing hemin-binding affinity and comparing it to known aptamers.
- Evaluating the peroxidase-like activity of hemin-aptamer complexes.
- Developing a chemiluminescence assay for nucleolin detection using AGRO100.
Main Results:
- AGRO100 and T30695 exhibit strong binding to nucleolin and HIV-1 integrase, respectively, and high hemin-binding affinity.
- Hemin-DNA complexes of AGRO100 and T30695 show superior peroxidase-like activity compared to PS2M.
- AGRO100 was successfully applied for sensitive chemiluminescence detection of surface-expressed nucleolin on HeLa cells.
Conclusions:
- Multifunctional G-quadruplex aptamers possess significant potential for biochemical applications.
- Exploiting new functions of existing aptamers can extend their utility in diagnostics and assays.
- The developed method allows for sensitive detection of specific proteins on cell surfaces.
