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Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
Published on: November 1, 2017
Differentiation of reprogrammed somatic cells into functional hematopoietic cells
Shengchang Huang1, Jianle Wang, Sheng Liu
1National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, PR China.
Differentiation; Research in Biological Diversity
|July 31, 2009
Summary
Somatic cell nuclear transfer (SCNT) reprogrammed testicular Sertoli cells into pluripotent stem cells. These reprogrammed cells demonstrated equivalent hematopoietic differentiation potential as fertilization-derived ES cells in vitro and in vivo.
Area of Science:
- Stem cell biology
- Reproductive biology
- Hematopoiesis
Background:
- Differentiated somatic cells can be reprogrammed into pluripotent stem cells.
- Reprogrammed cells can differentiate into various cell types, offering potential for cell therapy.
Purpose of the Study:
- To reprogram testicular Sertoli cells into pluripotent stem cells using SCNT.
- To investigate the hematopoietic differentiation potential of these reprogrammed cells.
- To compare their potential with fertilization-derived ES cells.
Main Methods:
- Somatic cell nuclear transfer (SCNT) was used to reprogram Sertoli cells.
- In vitro hematopoietic differentiation into embryoid bodies (EBs).
- In vivo transplantation into irradiated mouse bone marrow.
- Assessment of hematopoietic gene expression and colony-forming cell generation.
Main Results:
- Reprogrammed Sertoli cells (NT-ES) efficiently differentiated into hematopoietic EBs.
- Key hematopoietic genes (FLK-1, Bmp4, Runx1) showed dynamic expression during differentiation.
- Transplanted cells differentiated into functional hematopoietic lineages in vivo.
- Similar efficiency in generating blast-colony-forming cells (BL-CFCs) compared to F-ES cells.
Conclusions:
- Reprogrammed Sertoli cells possess equivalent hematopoietic differentiation potency as fertilization-derived ES cells.
- SCNT-derived pluripotent stem cells offer a viable alternative cell source for regenerative medicine.
- This study validates the potential of reprogrammed somatic cells for generating functional hematopoietic cells.
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