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Updated: Jun 20, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
Synthesis and characterization of deoxyuridine triphosphates labeled with pyrene
Yosuke Tanimizu1, Tadao Takada, Mitsunobu Nakamura
1Graduate School of Engineering, University of Hyogo, Himeji, Hyogo 671-2280, Japan.
Abstract:
Deoxyuridine triphosphate derivatives modified with pyrene was synthesized to functionalize DNA with fluorescent molecules based on the template DNA sequence. Incorporation of pyrene-labeled deoxyuridine triphosphates into DNA by DNA polymerase was investigated by using reverse-phase HPLC and polyacrylamide gel electrophoresis. The fluorescent properties of functionalized DNA were characterized by the steady-state fluorescence measurements.
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