The environmental effect on the fluorescence intensity in solution. An analytical model

Area of Science:

• Photochemistry
• Physical Chemistry
• Analytical Chemistry

Background:

• The fluorescence intensity of a fluorophore is influenced by its surrounding medium.
• Understanding these non-specific interactions is crucial for quantitative analytical applications.

Purpose of the Study:

• To propose a mathematical model describing the effect of the surrounding medium on fluorophore fluorescence intensity.
• To relate fluorescence intensity to solvent properties for analytical purposes.

Main Methods:

• Developed a model based on the energy gap law and non-radiative decay constants.
• Incorporated the effects of solvent dielectric constant and refractive index on energy gaps.
• Related fluorescence intensity (F) to solvent properties and solute concentration.

Main Results:

• The model establishes a relationship between fluorescence intensity and solvent dielectric constant and refractive index.
• A specific model was derived for solvent-solute systems, linking fluorescence to solute concentration.
• Initial experimental data support the model's validity.

Conclusions:

• The proposed mathematical model effectively describes medium-fluorophore interactions influencing fluorescence.
• The model provides a framework for quantitative analysis using fluorescence intensity.
• Solvation effects on energy levels and decay constants are key to predicting fluorescence intensity.