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Updated: Jun 19, 2026

Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy
Published on: April 3, 2017
STUDIES ON THE PROBLEM OF PRESERVATION OF MYELIN SHEATH ULTRASTRUCTURE: EVALUATION OF FIXATION, DEHYDRATION, AND
R M Condie1, A E Howell, R A Good
1Pediatric Research Laboratories of the Variety Club Heart Hospital, University of Minnesota, Minneapolis.
Abstract:
Currently accepted methods of tissue preparation for electron microscopy result in alterations of myelinated nerve fibers. In an attempt to minimize distortion of myelin, various fixation techniques, dehydration schedules, and embedding methods have been evaluated. It was found that the major damage to myelinated nerves occurs in the embedding procedure. A technique for embedding nerve tissue using the polyester Vestopal W is described which was found to result in improved preservation of myelin.
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The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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