Related Experiment Video
Updated: Jun 19, 2026

14:10
Video-rate Scanning Confocal Microscopy and Microendoscopy
Published on: October 20, 2011
Micromachined scanning confocal optical microscope.
Optics Letters
|October 31, 2009
Summary
We developed a miniature confocal microscope using fiber illumination and a zone plate lens. This compact device achieves 1.0-micrometer resolution for monochromatic imaging applications.
Area of Science:
- Optical Engineering
- Microscopy
- Nanotechnology
Background:
- Confocal microscopy is crucial for high-resolution imaging.
- Miniaturization of optical systems is a key challenge in modern instrumentation.
Purpose of the Study:
- To construct a compact, side-looking confocal optical microscope.
- To achieve high resolution in a miniaturized imaging system.
Main Methods:
- Utilized single-mode fiber illumination.
- Employed a two-phase off-axis zone plate objective lens.
- Integrated micromachined silicon torsional scanning mirrors for beam steering.
Main Results:
- Achieved 1.0-micrometer resolution.
- Demonstrated imaging over a 100-micrometer field of view.
- The objective lens has a numerical aperture (N.A.) of 0.24 at lambda = 0.6328 microm.
Conclusions:
- The developed miniature confocal microscope offers high resolution in a compact form factor.
- This technology is suitable for monochromatic imaging applications requiring miniaturization.
Related Concept Videos
Confocal Fluorescence Microscopy
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Overview of Microscopy Techniques
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Phase Contrast and Differential Interference Contrast Microscopy
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Imaging Biological Samples with Optical Microscopy
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Three-Dimensional Microscopy in Microbiology
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Atomic Force Microscopy
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

