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Harvesting and Disaggregation: An Overlooked Step in Biofilm Methods Research
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Tissue disaggregation
1Department of Biophysics, Armed Forces Institute of Pathology, Washington, DC, USA.
Methods in Molecular Biology (Clifton, N.J.)
|December 17, 2009
Abstract:
A conventional flow cytometry procedure for creating single cell suspensions is provided. Methods of tissue disaggregation to create single-cell suspensions from formalin fixed tissue are also given. Chemical, mechanical, and enzymatic multistep methods for separating single cells from tissue are discussed. These procedures are very tissue dependent.
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